| 1 |  Andre Tongo  Kim Garcia Ryan Romich Vanessa Conti | U40 | 03:48 | |
| 2 | Antonio Castro Jose Checo Kara Arbadji Kathleen Karpicki | U40 | 03:50 | |
| 3 | Beau Meredith Bianca Toledo COURTNEY ROSELLE Nick Music | U40 | 03:56 | |
| 4 | John Marshall  Micha Thaddeus Oliver Bentsen Susie Mulder | U40 | 03:57 | |
| 5 | Brittany Reed Chelsea Kadak Kevin Kadak Trevor Reed | U40 | 04:00 | |
| 5 | Chris Pytlowany  Evie Kaden Kevin Faranetta Melissa McKenna | U40 | 04:00 | |
| 7 | Christina Muccio Conor Murphy Marguerite Lee Tim Ferraro | U40 | 04:03 | |
| 8 |  Alejandro Rojas Alexandra Rodriguez Daniel Mora Sabrina Azocar | U40 | 04:04 | |
| 9 | Carlos Jimenez Evelyn Osio Marysabelle Everon Sebastian Rico | U40 | 04:08 | |
| 10 | Dylan Roche Emily Hard Olivia Murray Riley Doherty | U40 | 04:10 | |
| 11 | Emily Banerjee Frankie Rodriguez McKenzie Lyne Nick Williamson | U40 | 04:11 | |
| 11 | Emma Donchi Hadley Middelton Isaiah Mathieu Jake Cummings | U40 | 04:11 | |
| 13 | Francie Foley Jack Strum John Montenegro Karinne Couture | U40 | 04:12 | |
| 13 | Kevin Mulcahey Ryan Dudley Sarah Dudley Sarah Thomas | 40+ | 04:12 | |
| 15 | Adam Burns Lester Parmar Shelby Burns Tracy Parmar | U40 | 04:13 | |
| 16 |  Clara Nolasco  Kathe Bermudez Mario Bermudez  Marvin Rodriguez | 40+ | 04:21 | |
| 17 | Ali Alsaadi Darren Wagner Hannah Duffy Sarah Killoy | U40 | 04:23 | |
| 17 | Abbey Wolff James McNeer Nicole McNeer William Meijer | U40 | 04:23 | |
| 19 | Danny Carter Liana Tellez Paul Carter Theresa Carotenuto | U40 | 04:24 | |
| 19 | Conor Morford Crystal Boucher Keli Morford Tom Scappaticci | 40+ | 04:24 | |
| 21 | Jackie Greer Joe Reynolds Justin Messina Melissa Flanagan | U40 | 04:25 | |
| 22 | Joe Doherty Kim Doherty Megan Lifshitz Mike Lifshitz | 40+ | 04:26 | |
| 23 | Alyssa Honaker Katelyn Andrews Matthew Chaggaris  Renan De Oliveira | U40 | 04:31 | |
| 24 | Brittani Hetyei Claudio Morales-Perez Kelsey Mclean  Roberto Calitri | U40 | 04:32 | |
| 25 | Darren Hunt Dominic Picca Emma Downey Olivia Downey | U40 | 04:35 | |
| 26 | Andrew Lamson Bethany Rodriguez Mallorie Nai Ryan Rodriguez | U40 | 04:37 | |
| 27 | Benjamin Gradle Isabelle Rivera Kelly Gottlieb Ryan Cassidy | U40 | 04:38 | |
| 28 | Adam Singer Jeff Boudreau Lisa Szymanski Patricia Banker | 40+ | 04:39 | |
| 29 | Dalton Kamens Harvyn Valentin Hillary Valentin Sarena Boulier | U40 | 04:41 | |
| 29 | Amy Boyd Bryan Jones Shane Plunto Staci Portale | 40+ | 04:41 | |
| 29 | Alex Morin Amanda Libby James Hadley Lauren Bryant | U40 | 04:41 | |
| 32 | Julia Hausmann Karl Martin Miguel Goncalves Nicole Taranto | 40+ | 04:42 | |
| 33 | Dana Cooper Kenneth Fujita Maria Rodriguez Nick Vagelatos | U40 | 04:44 | |
| 33 |  Alexandra Bentos Dahiana Alzate Julian Rivillas  Sandro Cuesta | 40+ | 04:44 | |
| 33 | Haley Callahan Jessica Erasmi Kevin Murphy Patrick Lydon | U40 | 04:44 | |
| 36 | Allison Giorgio Eric Rapisarda Mel Ockerby Mike Giorgio | 40+ | 04:45 | |
| 36 | Alyssa Sullivan Cristian Morales John Borges Kristy Thompson | U40 | 04:45 | |
| 36 | Aleksandra Syssoev Kellyn Mcnamara  Kosta Hitollari Sean Mcnamara | U40 | 04:45 | |
| 36 | Daniel Santos Jacobo Bermudez Natali Zapata Sara Tabares | U40 | 04:45 | |
| 40 | Christopher Curtin Katherine Patnaude Keri Lloyd Matt Palazzo | U40 | 04:46 | |
| 41 | Brittany MacDonald Caroline Fedele Franklin Jose Uday Thonangi | 40+ | 04:52 | |
| 42 | Elyse Fannon Jessica Hall Mark Imber Stephen Gleason | 40+ | 04:54 | |
| 43 | Andrew Baker Brad Czarnec Brianna Roath  Jennifer Flynn | U40 | 04:55 | |
| 44 | Amanda Britko Colin Mccabe Dom Massey Peyton Madigan | U40 | 04:56 | |
| 45 | Jody Yang Jordan Hansen Matt Moore Morgan Harr | U40 | 04:58 | |
| 45 | Brent Lowell Keiahna Cargill Nelson Valencia Taisia Silva | U40 | 04:58 | |
| 47 | Anna Kay Chris Veilleux Kelly Healy Pat Hayes | U40 | 05:02 | |
| 48 | Brendan Morrissey Megha Sethi Rahul Sethi Suzanne Currie | 40+ | 05:06 | |
| 48 | Henry Martin  Jasmine Variam Leora Sanders Ronan Cunningham | U40 | 05:06 | |
| 50 | Ashlyn Howard Colleen Dalton Robert Raitano Jr Samwel Gitundu | U40 | 05:07 | |
| 51 | Chad Giannuzzi Kelly Giannuzzi Leslie Forker Vince Scacchitti | U40 | 05:11 | |
| 51 | Cody Cahoon Garrett Dobson Morgan Carson Taylor Casey | U40 | 05:11 | |
| 51 | Christopher Valorose Deanna Mazzei Hernando Rodriguez Rachel Marquebreuck | 40+ | 05:11 | |
| 54 | Connor Jones David Easterhoff  Tianyi Zhou | U40 | 05:12 | |
| 54 | Ashley Case Douglas Case Kerri Provencher Ryan Provencher | 40+ | 05:12 | |
| 56 | Benjamin Ryan Cameron Mastromarino Leah Mcginley Megan Ryan | U40 | 05:13 | |
| 57 | Emilia Gonzalez Gonzalez Joanna Kwiatek  Jorbis Feliz Prabh Singh | U40 | 05:14 | |
| 58 | Jennifer Mata Kevin Salazar Luis Romero Nicole Lopez | U40 | 05:15 | |
| 58 | Alexandra Kyriakides Alexa Nutter Alex Mikalajczyk William Morrison | U40 | 05:15 | |
| 60 | Alexa Andersen Bri Cain Garrett Molea Nicholas Piccola | U40 | 05:17 | |
| 61 | Abigail Miranda Angie Rieder Garrett Lapsys Matt Stahl | U40 | 05:19 | |
| 62 | Alex Stencel Grace Traboulssi Julia Paladino Samuel Stencel | U40 | 05:20 | |
| 63 | Brittany Ahlstedt Michael Padulsky Raphael Moraes Da Fonseca Taylor Cassidy | U40 | 05:24 | |
| 64 | Adrienne Maybin Kimberly Jefferson Patrick Jefferson Ron Hooks | 40+ | 05:26 | |
| 64 | Amy Shyu  Kuei Ting Chen Ying-Yu Lai Yiwei Lee | U40 | 05:26 | |
| 66 | Daniel Kenney Kristina Heffron Maria Kenney Michael Heffron | U40 | 05:28 | |
| 67 | Genaye Trimmer  Richard Parchment Sai Ruthvick Yuthika Maganti | U40 | 05:29 | |
| 67 | | U40 | 05:29 | |
| 69 | Alan Swierczynski Dan Tao Larissa Michel Lily Wetherington | U40 | 05:30 | |
| 70 | Fangyuan Zhao  Qian Wan William Tseng  Zheng Wei Tan | U40 | 05:31 | |
| 70 | Christine Daigle Jeff Bucci Jenn Kirkland Scott Redfern | U40 | 05:31 | |
| 72 | Cheryl Roucken Joe Roucken Katherine Mastroianno Rick Mastroianno | 40+ | 05:33 | |
| 73 | Caitlin Mirabell Daniel Hughes James Szymanski Marissa Beaton | U40 | 05:35 | |
| 74 | Ariel Lafreniere Deandrae Brown Kenny Stahovish  Zedekiah Maldonado | U40 | 05:38 | |
| 74 | Beau Claiborne Dan Biggers Malia Claiborne Teah Claiborne | U40 | 05:38 | |
| 74 | Alana Burke Hannah Mueller Tyler Bryant Zach Lambert | U40 | 05:38 | |
| 77 | Brenna Bowler Jesus Arocho Jesus Arocho Sr. Kealeigh Jenness | 40+ | 05:39 | |
| 78 | David Rodrigues Eliza Jackson Kyle Alexander Reba Hatcher | U40 | 05:40 | |
| 79 | Cormic Mcnamara Massimo Mitchell Rayhona Jafarzadeh Romina Jafarzadeh | U40 | 05:48 | |
| 80 | Dan Thurlow Kean Zimmerman Saige Bargon-Bourassa Sarah Rocco | U40 | 05:49 | |
| 81 | Dennis Trinh Jennifer Leshin Leah Shaffer Shamik Perry | 40+ | 05:50 | |
| 82 | James Brock Kristin Sinclair Megan Mahoney Teddy Salgado | 40+ | 05:51 | |
| 83 | Ashley Chambers Darin Daniel Julia Williams Scott Righini | U40 | 05:53 | |
| 84 | David Ng Harry Torres Kerry Guzman Laurie Santiago | 40+ | 05:58 | |
| 85 | Collin Doyle Jack Afanasiw Julia Dessart Kylee Dinneen | U40 | 06:01 | |
| 86 | Gavin Clark Heather Schon Jean Patino Kylee Vitka | U40 | 06:04 | |
| 87 | Joe Breen Kat Storey Tess Rose Tobore Emede | U40 | 06:05 | |
| 87 | I-Yang Chen Shenpeng Li Xiaoyun Pan Xuedi Yu | U40 | 06:05 | |
| 89 | Alexandra Schoolcraft Benton Carr Peter Midura Sufana Al Khunaizi | 40+ | 06:06 | |
| 90 | Kristen Gervasi Lauren Hurn-Bowling Max Murphy Richard Kingery | U40 | 06:13 | |
| 91 |  Cuong Huynh Kelyn Zhang Phung Vuong Toan Dao | U40 | 06:19 | |
| 92 | Beth Murphy Marissa Dimatteo Mike Leahy Tommy Dimatteo | U40 | 06:22 | |
| 93 | Jonathan Symons Naomi Lam Rachel Kane Ryan Rogers | U40 | 06:31 | |
| 94 | Bala Kailasam Kashish Gupta Narumanchi Gayatri Sampath Shantanu | U40 | 07:00 | |
| 95 | Cameron Carr Emma Carr Hayden Carr Madison Carr | U40 | 08:44 | |
