| 1 |  Amanda Sutton Kevin Gregory Matt Duval Melody Berrong | 40+ | 03:55 | |
| 2 | Eric Boone Jerry Spence Rebekah Langley Whitney Spence | U40 | 04:10 | |
| 3 | Brian Stewart Sharee Downs Terri Stewart Willie Freeman | 40+ | 04:26 | |
| 4 | Andrew Garritson Candice Brown Hannah Tatro Richard Pettus | U40 | 04:31 | |
| 5 | Dennis Owens Ethan Latimer Megan Robinson Missy Jones | U40 | 04:40 | |
| 6 | Eric Roberson Johnathan Carey  Monica Magcalas Raven McGregor | U40 | 04:42 | |
| 7 | Eli Jones Fayth Lasala Justus Hill Liberty Hill | U40 | 04:46 | |
| 8 | Allison Fisher Blake Ingram Madison Pollak Ryan Fraizer | U40 | 04:50 | |
| 9 | Jimmy Nixon Kathy Cheek KEVIN MARCY Stephanie Kull | 40+ | 04:53 | |
| 10 | Courtney Tucker Harry Buck Kennedy Wilson Malik Walker | U40 | 04:55 | |
| 11 |  Alan Huerta  Aliammar Malani Edith Maldonado Taylor Bordelon | U40 | 05:02 | |
| 12 | Ayden Hoang Chloe Carter Kim Hoang Kwakwu Kwarteng | U40 | 05:06 | |
| 13 | Buddy McGrath Evy Lewis Kyle Ference Rachel Trent | U40 | 05:09 | |
| 14 | Andrew Lockhart Ashlin Cosselman Courtney Roberts Padowithz Alce | U40 | 05:15 | |
| 14 | Isaac Jones Matt Green Montana Schug Oksana Dimitrova | U40 | 05:15 | |
| 14 | Janelle Moton Jasmine Holland Manny Moton Matthew Yancey | U40 | 05:15 | |
| 17 | Adrienne Hill Bree Pattillo Cal Abel Kris Funkhouser | 40+ | 05:17 | |
| 18 | Ashley Shaw Dakota Hooper Kelly Nguyen Luan Nguyen | U40 | 05:20 | |
| 19 | Chris Dunlap Erin Oglesby Stacy Nabor Tim Hanson | 40+ | 05:21 | |
| 19 | Amish Brahmbhatt Ava Grace Sestan Mackenzie Craig Shirish Vadali | U40 | 05:21 | |
| 21 | Angel Rodriguez Dariyen Naranjo Maricela Garibay Tracie Naranjo | U40 | 05:25 | |
| 22 | Katie Page Kiley Prevatte Rico Tungol Robert Calamas | U40 | 05:26 | |
| 22 | Antoine Smith Indya Tatum Kelly Dawsey Shannen Smith | U40 | 05:26 | |
| 24 | Darryl Haynes Gabriel Murphy LaReina Haynes Pedro Malu | U40 | 05:31 | |
| 25 | Emma Howatt Julia Winecoff Ron Utreras Yasin Lee | U40 | 05:35 | |
| 25 | Cheyenne Walsh  Fernanda Solano Mark McCourry  Mateus Brito | U40 | 05:35 | |
| 27 | Bert Griggs JAMES CIOFFI Mercy Rosario Stephanie Griggs | 40+ | 05:37 | |
| 28 | Jack Mariana Kendra Fuentes Laila Orlando Vinny Orlando | U40 | 05:38 | |
| 29 | Brian Tinnell Emma Nelson Gracie Mogelnicki Jace Nelson | U40 | 05:39 | |
| 30 | Ally Minson Chase Howell Jordan Minniefield Kate Lipa | U40 | 05:43 | |
| 31 | Baylor Aycock  Christina Kiessling Nicholas Barnett Robert Jefferson | U40 | 05:45 | |
| 32 | Ana Pimentel Briana Blake Carl Hood Jon Bartholomew | U40 | 05:56 | |
| 33 | Cesia Maldonado Matthew Muñoz Mehek Jan  Omar Neyazi | U40 | 06:10 | |
| 34 | Joshua Johnson Luke Elliott Mattie Johnson Megan Johnson | U40 | 06:43 | |
