| 1 |  Chris Beck Erin Antoniak Haley Smith Jesse Biddle | U40 | 03:34 | |
| 2 |  Ben Sawkins Colette Mofakham Dominic Stadnyk Holly Clark | U40 | 03:45 | |
| 3 | Ben Miller Evan Wick Maddie Miller Maddy House | U40 | 03:49 | |
| 4 | Dan Jonah Hannah Goncalves Kiefer Calisto Laura Somerville | U40 | 03:53 | |
| 4 | Christina Hale Edward Wheeler Gregg McLeod Janet Guerrette | 40+ | 03:53 | |
| 6 | Aaron Koeninger Ali Violand Brooke Koeninger Jimmy Violand | 40+ | 03:55 | |
| 7 | Adan Ibarra Alexis D’Souza Leah Hasson Lester Maxwell | U40 | 03:56 | |
| 8 | Alexander Golik Joseph Rucco Kelly Sullivan Lindsay Rucco | U40 | 03:57 | |
| 9 | Ally Tufano Kevin Donohue Madison Bartlett Matteo Tufano | U40 | 03:58 | |
| 9 |  Amy Orellana Dana Opeikun Josh Krantz  SEBASTIAN MEDINA | U40 | 03:58 | |
| 11 | Maddie Mills Matthew Kemp Myles Davis Sam Steiner | U40 | 03:59 | |
| 12 | Alexis Dreiss Daniel Weimar Gabriela Olivier Jose Carchi | U40 | 04:02 | |
| 12 |  Jack Parry Nicholas Anderson Sierra Baker Sophia Ryterband | U40 | 04:02 | |
| 14 | Alexandra Jennings James Jennings Joni Heidt Scott Bossert | 40+ | 04:03 | |
| 14 | Alexander Morris Daniel McGranaghan Lidia Henderson Michelle Scharfstein | U40 | 04:03 | |
| 16 | Andrew Jacobs Kelsey Lovelace Meghan McGowan Robert McGowan | U40 | 04:06 | |
| 17 |  Denny Wang Linda Lam Nicole Hu  sunvannak iv | U40 | 04:11 | |
| 18 | Alyssa Marotte Bryce Rawls Megan Brosnan Wyatt Moss | U40 | 04:13 | |
| 19 | Alex Schultz Keaton Spore Leigha wetter Terese Spore | U40 | 04:14 | |
| 20 | Alyssa Baggenstoss Rodrigo Marmol Sherri Smith Travis Rothstein | U40 | 04:15 | |
| 20 | Juan Giron Lacey Giannetta Megan Burns Tyler Haines | U40 | 04:15 | |
| 22 | Diego Devia  Josdy Gonzalez Karen Alvarez Paul Gómez | U40 | 04:17 | |
| 23 | Allison Mistrot Iain Mistrot Jeff Gibson Trisha Caton | U40 | 04:18 | |
| 23 | Catherine Webber John Jiang Sean Mcgrann Tiffany Sutherlad | 40+ | 04:18 | |
| 23 | Chungyen Tsai Daniel Zheng Jia Yi Nie  Jingxuan Lu | U40 | 04:18 | |
| 26 | Alexander Rachlin Helen Field Joseph Welch Noelle Li | U40 | 04:19 | |
| 26 | Abby Schutt Beckman Cody Sjodin Lisa Dehnel Ryne Reuvers | U40 | 04:19 | |
| 28 | Amy Bevilacqua James Caroland Jamison Spicer Liz Torres | 40+ | 04:20 | |
| 29 | Alekz Munoz Jessica Theriault Kerry Winfrey Sofie Trapp | U40 | 04:26 | |
| 30 | Christopher David Darien Giles Lillian Maltese Melissa Dieudonne | U40 | 04:27 | |
| 31 | Eyasu Moloro Jess McMillan Jonah Hetherington Maria Sinagra | U40 | 04:28 | |
| 31 | Juan Pablo Guarin Bermudez  Kirby Reyes Rosa Cedal Stephanie Delgado | U40 | 04:28 | |
| 33 | Christine Barnes Ermira Takla Fred Dimyan Rafik Takla | 40+ | 04:29 | |
| 34 | Amanda Raynor Emma Bogner Todd Wolffrum Wade Raynor | U40 | 04:30 | |
| 34 | Andria Winger Austin Winger Jonathan Kazor Kathleen Smith | U40 | 04:30 | |
| 34 | FANGZE ZHENG Tianpei Zhao Yiling Zheng Zinan Chen | U40 | 04:30 | |
| 37 | Bryan Parker Daniel Lee Mallory Ortman Rebecca Collins | 40+ | 04:31 | |
| 38 | Cristina Petroni Jessica Naroden  Kennard Wilson Jr Rudy Petroni | U40 | 04:32 | |
| 38 | Abigail Maines Derek McConnell Kyle Zufra Tj Goddard | U40 | 04:32 | |
| 38 | Andrew Lingao Kelli Finnegan Lauren Katzen Nolan Carbeck | U40 | 04:32 | |
| 41 |  David Costa Dayane Costa Karis Kim Wagner Costa | U40 | 04:33 | |
| 41 |  Ameer Shujjah Margaret Silliman Mayuran Chandrakanthan Sofia Capretta | U40 | 04:33 | |
| 43 | Camilla Mia Carag Carol Zhang Jonathan Ma Lucas Ruffel | U40 | 04:34 | |
| 44 | Ashley Lewis Cory Ranschau Kyle Dempsey Steve Sutman | 40+ | 04:36 | |
| 45 | Christopher Longo Gina Free Kelsey Glass Will Walsh | U40 | 04:37 | |
| 46 | Franny Spinosa Jessie Rivas John Narducci TeresaAnn Reta | U40 | 04:38 | |
| 46 | Amanda Rowe Jocelyn Lee Kenneth Chen Patrick Chan | U40 | 04:38 | |
| 48 | Armand Diesso Danielle Gogarty Pamela Lazarakis Raymond Gogerty | 40+ | 04:40 | |
| 49 | Edgar Iglesias James Reed Kristina Reed Nicole Dicroce | U40 | 04:41 | |
| 50 | Carl Colditz Maeve Healy Tiffany Caba Abreu Yuhung Leesheng | U40 | 04:46 | |
| 51 | Alexandra Johnson Anthony Pititto Francesca Pititto Vincent Pititto | U40 | 04:47 | |
| 51 | Nathaniel Schwab Shanxiao Zhang Xiaoyi Chen Yichen Wang | U40 | 04:47 | |
| 51 | Alex Yoon Charlene Chen emily Sanchez Enrique Ramos | U40 | 04:47 | |
| 54 | Chase Degasperis Emily Redgate Grant Holbrook Nicolette Depalma | U40 | 04:49 | |
| 55 | Jennifer Flynn Michelle Sheehan Nicholas Zaccaro Robert Marcotte | 40+ | 04:52 | |
| 56 | Eleanor Yip Emily Yip Justin Portes Ryan Green | U40 | 04:55 | |
| 56 | James Lavela Julia Vasconez Ryan Gribbon Vanesa Kim | U40 | 04:55 | |
| 58 | Breanna Edwards Chris Witcher John Edwards Sarah Oxholm | U40 | 04:57 | |
| 58 | Heather Milton Hunter Carter Jose Benitez  Melissa Mastoridis | U40 | 04:57 | |
| 58 | Damian Walls Molly Wingerd Sara Trout Thomas Jackson | U40 | 04:57 | |
| 61 | Edward Miller Jo Mailloux Michella Calix Patrick Robertson | U40 | 04:58 | |
| 62 | Christine Ko Hanaro Li Joel Choe Norman Young | U40 | 05:00 | |
| 62 | George Albert Grace Albert Jacquelyn Walker John-Aaron Hixson | U40 | 05:00 | |
| 64 | Art Clark Gaspar Gonzalez Giana Gonzalez Krystal Chapa | U40 | 05:01 | |
| 64 | Gregory Chrysler Hannah Lifshits Sami Tocman Stevie Bovo | U40 | 05:01 | |
| 66 | Catherine Bradley Chase Heichel Harry Parker Rachael Persky | U40 | 05:03 | |
| 67 | Deana DiSilvestre James DiSilvestre Michael Martin Nevele Martin | 40+ | 05:04 | |
| 68 | Frances Matte Joshua Kedzierski Peter Matte Sandra Rocca | 40+ | 05:05 | |
| 69 | Alexis Gillio Casey Schick Deborah Rose James Beyer | U40 | 05:07 | |
| 69 | Darline Kryta James Rathwell Joanna Rathwell  Mark Angsioco | U40 | 05:07 | |
| 71 | Alisha Schuck Koty Wong Vicki Fung Wing Keung Chin | U40 | 05:09 | |
| 72 | Miyu Ono Rachel Nazarian Tom Stratford  Willem Paans | U40 | 05:11 | |
| 72 | Jenna Zilincar Kelley Matthews Maggie Long Ryan Matthews | 40+ | 05:11 | |
| 72 | Cesar Lopez Nicholas Chen Olivia Rossi Rylee Phelps | U40 | 05:11 | |
| 75 | Andrew Leahy Deanna Bienkowski Justin Meyer Olivia Cerbo | U40 | 05:12 | |
| 75 | Ayana Davis Claron Johnson Matt Klamo Michelle Dipolvere | U40 | 05:12 | |
| 77 | Alyson Joubert Bryan Meseika Chris Joubert Stephanie Meseika | 40+ | 05:14 | |
| 77 | Ada Thatcher-James Dan Knapp Kelly Nachbar Luis Kumanduri | U40 | 05:14 | |
| 79 | Ameenah Little Anthony Solano Caylia Wallace Frederick Philp Jr. | U40 | 05:15 | |
| 80 | Aaron Long Dan Meinerz Rebecca Niziol Sarah Moraes | U40 | 05:17 | |
| 81 | Ben Blackwood Caroline Duckworth Dan Schaal Riley Rudd | 40+ | 05:18 | |
| 82 | Hanzhang Liu Haojian Zhang Sitao Zhang Tianheng Hu | U40 | 05:20 | |
| 83 | Christopher Lee Dillon Tjiptamustika Gina Grace Huh Kayla Chung | U40 | 05:21 | |
| 84 | Camila Hidalgo Nikhil Dondapati Phillip Li Yuqing Cao | U40 | 05:23 | |
| 85 | Alexander Trauber Emma Trauber Isabella Trauber Rustin Wiser | U40 | 05:24 | |
| 85 |  Christian Spetz Erika Fortune Selina Kemper Yi Liang | U40 | 05:24 | |
| 85 | Alan Joseph Caceres Kinzang Tobgay Liliana Torres Marygrace Tabing | U40 | 05:24 | |
| 88 | Brandon Dennis Brian Dennis Caroline Edwards Jordyn Martini | U40 | 05:25 | |
| 89 | Brandon Fleming Ciera Brown James Frieson Lakeshia Foster | U40 | 05:26 | |
| 90 | Antonio Diaz Jackie Walzer Katie Ellig Nate Assavarungnirund | U40 | 05:29 | |
| 90 | Brian Varela isabella kratz Kayla Murray Tanner Pflueger | U40 | 05:29 | |
| 90 | Laura Nguyen Linda Nguyen Michael Nguyen Paul Doan | U40 | 05:29 | |
| 93 | Amanda Cordero Caleb Johnson- Burroughs Franklin Nnamani Lorena Johnson- Burroughs | U40 | 05:32 | |
| 94 | Benson Chang Connie Hu Juan Diego Olivella Nilam Patel | U40 | 05:33 | |
| 95 | Devon Schmitt Ethan Avitia Ty Carpino Vanessa Bottos | U40 | 05:35 | |
| 96 | Jay Mai Jessica Moon Michael Song Peerapa Moolsintong | 40+ | 05:36 | |
| 97 | Alex Haag Antonia Gabriele Dan Saldanha Morgan McAtee | U40 | 05:37 | |
| 98 | Eileen Lofrese Lauren Tauro Patrick Raymond Ronald Lofrese | U40 | 05:38 | |
| 99 | Bruce Biersack Christina Gerdes  Marcel Hostin Samantha Biersack | U40 | 05:40 | |
| 100 | Corey Nocco Kali Blocker Shannon Washington Thomas A. Harris | 40+ | 05:43 | |
