| 1 |  Lucian Ficau Naomi Morris  Nolan Fredericks  Sarah Moloney | — | 03:40 | |
| 2 |  Alessia Donati Giovanni Benzon Gloria Corbetta Salvatore Scolaro | — | 03:47 | |
| 3 | Chris Bexon Claire Oliver Matt Oliver Nicola Bexon | — | 03:55 | |
| 4 | Benedetta Caporicci Cristiano Olivieri Gabriele Tordi Melania Fiordi | — | 04:00 | |
| 4 | Colm Mulryan Dean Nolan Louise Quinlan Rebecca Nolan | — | 04:00 | |
| 6 | Debora Tamburrieri Elena Fiorucci Francesco Filippo Gerli Mirsad Bajrami | — | 04:04 | |
| 7 | Alessio Erra Filippo Della Latta Myriam Grassi Ylenia Donati | — | 04:05 | |
| 8 | Andrea Giovanazzi Giorgia Taini Ludovica Raggi Massimiliano Giacomo Dominici | — | 04:10 | |
| 9 | Alessandra Paradiso Andrea Falone Fabrizia Tagliente Simone Firmani | — | 04:12 | |
| 10 |  Andre Curmi Emmanuel Mangion Joanne Lewis Raissa Camilleri | — | 04:14 | |
| 11 |  Darcie O’Connell Justin Liang Muhammad Alshamkhi  Nour Saad | — | 04:15 | |
| 12 | | — | 04:17 | |
| 13 | Adam Duncan-Rusk Caitlin King Gareth Fenney Laura Pitt | — | 04:21 | |
| 14 | Claudia Di Gennaro Fabiana Pepe Ruggero Lombardi Valerio Ferraro | — | 04:28 | |
| 15 |  Carla Da Silva Elisa Da Silva Manuel Da Silva Marine FURNON | — | 04:29 | |
| 16 | Alex NEMER Constance DOLO Frédéric Vallet Virginie PIGEAT | — | 04:35 | |
| 17 | Emre Korkmaz Julien FINES Prathana BOUNSING Varouny INTHAVONG | — | 04:37 | |
| 18 | Alessandra Tommasini Andrea Dezi Paolo Tommasini Valeria Toraldo | — | 04:44 | |
| 19 | davide cordovana umberto oddo veronica sotera vittoria vecchio | — | 04:46 | |
| 20 | Aurélie LAINE Dimitri TORCHIA Hugo PATOUT Lucile Michaux | — | 04:49 | |
| 20 | Aaron Brough Ellie Price Sarah Garrett Simon Garrett | — | 04:49 | |
| 22 | Antonio Di Maio DANIELA BONOMO PIETRO ANGELO BONOMO ROSANNA MESSANA | — | 05:03 | |
| 23 | Alexis Verderi Alix Gander  Carina Stöttner Josef Botes | — | 05:04 | |
| 24 | Clarisse MOULIN Maxime GILLOT Pauline Lepage Vincent BLANDIN | — | 05:08 | |
| 25 | Debora Valenti Francesco Fiori Nicholas Fresch Silvia Santo | — | 05:12 | |
| 26 | Margaux WALUKIEWICZ Mathieu GONTIER Sandra CHETANEAU Yazid Mesbahi | — | 05:24 | |
| 27 | Elisabetta Calicchio Elisa Committeri Matteo Cifonelli Michele Brilli | — | 05:29 | |
| 28 | Charline LEVILLAIN Etienne PEROL Gabriela RANDRIAKOTONIRINA Nicolas SANSAC | — | 05:30 | |
| 29 |  Greta Kungė Laura Šupailienė Modestas Kungys Saulius Supaila | — | 05:41 | |
| 30 | Alberto Botticelli Andrea Rubino Mara Mazzocco Nicole Zerega | — | 05:42 | |
| 31 | Francesco Rachiglio Martina Clementi miriana carlini Simone Pizziconi | — | 05:51 | |
| 32 | Angelo Lo Cascio Erica Franco  Francesco Barresi Martina Mancini | — | 06:33 | |
