| 1 |  Carolyn Ortega Ceasar Mendros Javier Carvajal Jessica Lowery | U40 | 02:54 | |
| 2 | Carly Wilkins Matthew Cosgrove Megan Horn Sam Pascale | U40 | 04:11 | |
| 3 | Alexis Decamillo Bo Schlechter Deandres Jackson Jessica Schlechter | U40 | 04:17 | |
| 3 | Dextina Holt Gary Davis Sadeliah Moody Victor Moody Jr | U40 | 04:17 | |
| 5 | James Ross Jeanine Foster Luis Puga  Samantha Flores | U40 | 04:20 | |
| 6 |  Andrés Piske Armando Taoli Catheryne Tovar Maria Fabiana Reigadas | U40 | 04:24 | |
| 7 | Eric Roberson Johnathan Carey  Monica Magcalas Raven Mcgregor | U40 | 04:31 | |
| 7 | Cheyn Bartels Chris Clouse Emily Martinez Katelynn Tucker | U40 | 04:31 | |
| 9 | Alex Frank Lacey Sorrentino Michael Cooley Todd Payne | 40+ | 04:36 | |
| 10 | Daniel Derasmo Derrick Smielewski Jordan Devereaux Leonie De La Cruz | U40 | 04:40 | |
| 11 | Finn Richert Kate Richert Maddie Richert Will Richert | U40 | 04:41 | |
| 12 | Carlos Herrera Dorianys Mirabal Jonathan Hernandez  Luiza Albertim | U40 | 04:42 | |
| 13 | Daniel Williams Elizabeth Hayne John Kelly Kailynn Violette | U40 | 04:44 | |
| 14 | Amy Clement Daniel Wolf Gabriel Diaz Jessica Garcia | U40 | 04:46 | |
| 15 | Christopher Williams Davis Frank Jillian Williams Kasia Szymanska | U40 | 04:49 | |
| 16 | Chris Strauss Lauren Paneyko Mike Anziano Sasha Gerbrandt | U40 | 04:51 | |
| 17 |  Martin De Juan Matias Lozano Sabrina Lozano Sharon Berkley | U40 | 04:53 | |
| 18 | Jaime Fernandez Jim Mclaughlin Kellie Arteaga Nicole Culver | 40+ | 04:58 | |
| 19 | Darien Samuels Kenneth Cooper Maria Cassandra Montoya Marit Middendorp | U40 | 04:59 | |
| 20 |  Dana Garrod Ian Plante-Signal Jessica Perigo William Hand | U40 | 05:00 | |
| 21 | Jorge Torres Kayla Sadler  Maria Cognac Raymond Pulido | 40+ | 05:01 | |
| 22 | Alejandra Galindo  Jose Luque Juan Uribe Nadine Zyman | U40 | 05:11 | |
| 23 |  Kim Catenacci Lauren Almeida Luke Catenacci Miguel Sira | U40 | 05:14 | |
| 24 | Estelvy Bellorin Luis Bellorin Santiago Gamboa  Yolanda Gavillam | U40 | 05:16 | |
| 25 | Lorena Claure Maria Xing Sergio Claure | U40 | 05:22 | |
| 26 | Daniel A Plaza Gabriella Goes Karen Hidalgo Krista Haapalainen | U40 | 05:24 | |
| 27 | Aaron Bernstein Ghilan Golzar Jessica Maduro Sementilli Kimberly Steele | U40 | 05:25 | |
| 28 | Alejandro Forcade  Amalia Benitez Raideliz Parra Wendy Forcade | U40 | 05:26 | |
| 29 | Abigail Strecker Anthony Klingensmith Daniel Diloreto Madison Mccorkle | U40 | 05:28 | |
| 30 | Loni Siegers Patricia Lafond Patrick Lafond Scott Siegers | U40 | 05:34 | |
| 31 | Dana Biggi Jeff Holland Rick Potisto Wendy Holland | 40+ | 05:40 | |
| 32 | Dan Mihai David Leal Kristen Munson Tracey Woodson | U40 | 05:42 | |
| 33 | Billy Tierney Jason Hearty Rebecca Boucher Sejla Dolic | 40+ | 05:57 | |
| 34 | Derek King  Michelle Tran Ricardo Almonte Vanessa Villegas | U40 | 06:21 | |
| 35 | Anne Eddy Cory Sands Dana Sands Paul Gram | 40+ | 06:27 | |
| 36 | Erica Morales Jose Morales Mellissa Madrigal Savian Fernandez | U40 | 06:44 | |
| 37 | Anyla Fulton Jania Davis Mark Davis Steven Everson | U40 | 06:56 | |
