| 1 |  Keegan Peach Kerri Huntington Sabrina Daolio Simon Levin | U40 | 04:00 | |
| 2 | Casey Bekker Joshua Ruger Mike Fawcett Vicky-Leigh Kruger | U40 | 04:09 | |
| 3 | Aidan De Andrade Gina Grippa Robyn Bull Tristan Nienaber | U40 | 04:10 | |
| 4 | Michael Thackwray Paige Georgiades Simona Moi Wandile Zweni | U40 | 04:15 | |
| 5 | Cassidy Junius Gerhard Junius Isabel Junius Johann Junius | U40 | 04:18 | |
| 6 | Ellie Watkins JT Claassen Michael Terblanche Robyn Barnard | U40 | 04:23 | |
| 7 | Amanda Viljoen Clinton Dreyer Danelle Dreyer Hennie Viljoen | 40+ | 04:28 | |
| 8 | Dune Hunkin Hilton Hunkin Mariana Van heerden Pieter Willem Van Heerden | U40 | 04:30 | |
| 9 | Bianca de Beer Daniel Jardim Kyle Forsyth Shelby Forsyth | U40 | 04:31 | |
| 10 | Bianca Putter Chris Knez Michelle Knezovich Ricky Papale | 40+ | 04:33 | |
| 11 | Chris Lewis DINA POWER Jason Hall Raffaella Montali | U40 | 04:34 | |
| 11 | Grant Jacobsen Jacques Viljoen Jeanette Viljoen Robyn Jacobsen | 40+ | 04:34 | |
| 13 | Connor Tarabanov Marlene Axsel  Michaela Mitchell Zaundré Shortt | U40 | 04:39 | |
| 13 | Angela Mostert Debbie Muir Evan Kay-Clough Jonathan Muir | 40+ | 04:39 | |
| 15 | Amorie Stark Anthony Bird Fanie Smuts Zelma Bird | U40 | 04:56 | |
| 16 | Clintin Riddell Kerryn Hayter Marike Kruger Sean Mumford | U40 | 05:01 | |
| 17 |  Kiribakka Tendo Lebogang Hlatshwayo Precious Allessing Warren Allies | U40 | 05:05 | |
| 18 | Julie-Ray Vorster Len Cullen Nicky Coetzee Reuben Vorster | 40+ | 05:12 | |
| 19 | Bernhard Westra E-andri Swiegelaar Jaco Van Wyk  Kayla De Wet | U40 | 05:14 | |
| 20 | Christelle McMurtry Riaan Le Roux tracey herholdt Waldt Human | U40 | 05:22 | |
| 21 | Adhil Somai Candice Brakhoven Natasha Van der Walt Vincent Heyns | U40 | 05:29 | |
| 22 | Bradley Khoza Karabo Morake Makhosazana Thom Sarah Mading | U40 | 05:33 | |
| 23 | Katlego Matsheka Mariska Munro Nandi Buthelezi Zwelithini Mbele | U40 | 05:41 | |
| 24 | Boipelo Molefe Buko Fortein Sipho Mseleku Thato Tloti | U40 | 05:51 | |
| 25 | Hlomendlini Tshabalala Nandi King Nhlakanipho Phakathi Nomampondomise Belot | U40 | 05:53 | |
| 26 | BOHANG MOEKO Candice Leigh Coulsen Jenna Emily Rivera TULANI NKAZIMLO MADALA | U40 | 06:08 | |
| 27 | Dakalo Nemasetoni Mpho Nemasetoni Mulalo Nemasetoni Rudzani Nemasetoni | U40 | 06:18 | |
| 28 | Celia Potgieter Christelle Van Biljon Francois Schlebusch Peet Meyer | 40+ | 06:31 | |
