| 1 |  Chastity Singh James Boen Jason Adams Kaytee Veracruz | U40 | 03:51 | |
| 1 | Lalo Contreras Lauren Inmon Mercedes Boucher Neil Krebs | U40 | 03:51 | |
| 3 | Chris Balven Derek Nelson Kara Mobly  Maria Fecik | U40 | 03:54 | |
| 4 | Bryce Anderson Kelsee Benge Kourtney Small Taylor Small | U40 | 04:00 | |
| 5 | Cooper Mery Kulani Subiono Mak Isbill Savannah Kepler | U40 | 04:03 | |
| 6 | Keri Cunnane Raven Rodriguez Tomas Sardina Wayne Brunet | U40 | 04:12 | |
| 7 | Joe Lowry Nyddia Hannah Ryan Calhoun Sasha Jones | 40+ | 04:17 | |
| 8 | Hattie Lankford Jen Barnes Nick Alaniz Zia Ali | U40 | 04:20 | |
| 9 | Dennis Garcia Hailey Marie Maria Hartstein Sam Auge | U40 | 04:24 | |
| 10 | Austin Hogan Bernie Gutierrez Courtney Levering Kwabe Obeng | U40 | 04:29 | |
| 11 | Bryan Hamren Chunyi Wang Joaquin Feliciano Yuntao Wang | U40 | 04:31 | |
| 12 | Elise Blount Omar Jenkins Sara Ahmad Stephen Pine | 40+ | 04:48 | |
| 13 | Bryan Elmer Edward Krische Racheal Minor Robyn Ahrens | U40 | 04:51 | |
| 14 | Caitlynn Sutton Jamison Ethington Jeannie Kubricht Ryan Veariel | U40 | 04:54 | |
| 15 | Dawn Rayls  Richard Tijerina Ryan Ridder Stephanie Ridder | 40+ | 04:59 | |
| 16 | Deyaun Seale Felipe Jimenez Maricruz Vasquez Olamilekan Ajala | U40 | 05:06 | |
| 17 | Carlos Arce Kristin Meyer Laury Arce  Matthieu Barbosa | U40 | 05:09 | |
| 18 |  Jared Avery Jason Sergent Victoria Holt Yadira Moya | U40 | 05:13 | |
| 19 | Connor Brem Erin Contreras Heath Shifflett Kristel Wiltz | U40 | 05:15 | |
| 20 | Dave Neely Jeff Mcelderry Joanne Mcdonald Michelle Booker | 40+ | 05:30 | |
| 21 | Jacob Mury Kate Russell Kendra Elder Luke Froehlich | U40 | 05:35 | |
| 22 | Jason Boorstein Magali Gomez Mark Frenkel Shelly Reynolds | 40+ | 06:01 | |
| 23 | Andrew Knust Jose Vega Valerie Wilson Vanessa Gonzalez | 40+ | 06:09 | |
| 24 |  Chad Wilkinson Desiree Cortez Heidi Jo Medina Mathew Kite | U40 | 06:30 | |
| 25 | Beverly Smith Jeff Smith Lindsay Abstein Talon Smith | 40+ | 06:51 | |
