| 1 |  Jonny Hunt Laurel Macgregor Nicole Lanning Noah Weeks | U40 | 03:45 | |
| 2 | Bridget Brown Corey Brown Matt Stull Mollie Stull | U40 | 03:54 | |
| 3 | Alayna Dufour Loren Newman Paul Holmer Robert Wittenauer | U40 | 04:00 | |
| 4 | Adriana Stavreva Donald Kidwell Kamila Malewicki Katherine Madden | U40 | 04:02 | |
| 5 | Cj Dick Jennifer Halloran Jojo Farella Shaina Mae Rowley | U40 | 04:03 | |
| 6 | Abigail Quirin Carter Yang Geetanjali Scarff Parker Weiss | U40 | 04:05 | |
| 7 | Ashley Cunningham Brad Gunderson Kristin Basso Rusty Mcconnell | U40 | 04:10 | |
| 8 | Kayla Degeorge Mario Figiola Samantha Wilcko William Heilman | U40 | 04:11 | |
| 9 | Elizejoy Guzzarde Michael Guzzarde Monique Pineda Rendell Pineda | U40 | 04:12 | |
| 10 | Bonita Cho Ernest Sherman Hailey Cho Michael Cho | 40+ | 04:24 | |
| 11 |  David Chriswick Justin Thomas Kate Fowell Katherine Hunt-Morr | 40+ | 04:25 | |
| 12 | John Gallego Juan Carlos Hernandez Karen Suarez Maria Bravo | 40+ | 04:26 | |
| 13 | Al Waterloo Joan Richards Lieke Van Heumen Pat Nolan | 40+ | 04:33 | |
| 14 | Alexandra Bochis Matt Nakachi Mike Miles Vicki Dallas | 40+ | 04:39 | |
| 15 | Carolyn Revilla Elias Paulos Pete Paulos Tremaine Gordon | U40 | 04:47 | |
| 16 | Adriana Soto Billy Papadimas Lauren Biazzo Mike Dietrich | U40 | 04:48 | |
| 17 | Aaron Christensen Grant Kuchan Joe Gerbasi Kristen Chau | 40+ | 04:53 | |
| 18 | Amber Isaac Jennifer Murzyn Patrick Isaac Timothy Smith | 40+ | 04:55 | |
| 19 | Anastasia Stanger Jay Dobbs Paul Anderson Robyn Kaufman | U40 | 04:59 | |
| 20 | Adam Rallis Andrew Rallis Joanne Moreno Rebecca Rallis | 40+ | 05:00 | |
| 21 | Brooke Byas Julia Callahan Sean Pankuch Virgil Cannon | U40 | 05:05 | |
| 22 | Anita Clifford Charles West Isaias Valladares Kristi Nickless | U40 | 05:08 | |
| 22 | Anne Root Becraft Kiley Mccown Mark Schwieterman Stacy Mancl | 40+ | 05:08 | |
| 24 | Carmilla Kupritz Joaquin Pratt Leo Montano Neena Denne | U40 | 05:12 | |
| 25 | Alex Fernandez Gina Skiera Jessie Kilavos Mike Shimizu | U40 | 05:13 | |
| 26 | Antwine Duffy Matthew Maclean Natalie Schlicht  Polina Melnik | U40 | 05:18 | |
| 27 | Alex Wolstenholm Ashlyn Wolstenholm Becky Bitar Luis Arteaga | U40 | 05:21 | |
| 28 | Andrew Roth Christine Roth Mike Roth Stephanie Ottosen | U40 | 05:23 | |
| 29 | Adriana Ziza Beth Grinter Jane Shaughnessy Jason Nagy | U40 | 05:27 | |
| 30 | Brodie Schultz Megan Ziegler Paul Vance Shawn Walkiewicz | U40 | 05:28 | |
| 31 | Julyana Agrelo Magali Depilla  Sandro Sanchez Vasilia Garbis | U40 | 05:31 | |
| 31 | Alfredo Briseno Carlos Zamorano Laveena Dsouza Megan Mf Kelleher | 40+ | 05:31 | |
| 33 | Lexie Blaner Lorena Hernandez Lucy Placzek Robert Washington | U40 | 05:32 | |
| 33 | Carlos Luciano Jeffrey Moeller Pooja Sharma Shara Lasoff | U40 | 05:32 | |
| 35 | Denys Delgado Phong Nguyen Sandra Medina Stephanie Ortiz De Villate | U40 | 05:35 | |
| 36 | Jen Hayduk Jess Walpole Scott Hayduk Tom Walpole | 40+ | 05:38 | |
| 37 | Brynn Vish Matt Vish Natalie Weekley Steve Snyder | U40 | 05:41 | |
| 38 | Diana Bahena Harshil Patel Natalie Kirykowicz Roman Dobkowski | U40 | 05:44 | |
| 39 | Joe Lappay Marcus Hall Megan Hall Melanie Baronas | U40 | 06:03 | |
| 39 | Josiah Novak Michelle Novak  Rithesh Ram Veronique Ram | 40+ | 06:03 | |
