| 500 |  Austin Maser Kaija Bramwell-Maser | 16-29 | 04:25 | |
| 500 | Chris Norman  Marianela Porras | 30-39 | 04:25 | |
| 500 | Anna Ward  Javion Green | 30-39 | 04:25 | |
| 500 | Cameron Evans Yash Patel | 16-29 | 04:25 | |
| 500 | Alpha Diane Okenna Okpareke | 30-39 | 04:25 | |
| 500 | Devin Weilbaecher Michael Wilson | 16-29 | 04:25 | |
| 500 | Anders DeNeergaard Nick Nasr | 16-29 | 04:25 | |
| 500 | Doug Chandler Phil DeMonte | 50-59 | 04:25 | |
| 509 | Jimmy Glover Taylor Glover | 50-59 | 04:26 | |
| 509 | Daniel Estes Paige Payne | 16-29 | 04:26 | |
| 509 | Chad Brown Dan Eipp | 30-39 | 04:26 | |
| 509 | Jessica Cantrell Jonathan Sams | 16-29 | 04:26 | |
| 509 | Michael Adams  Rishabh Bhandawat | 30-39 | 04:26 | |
| 509 | Caleb Odom Olivia Noland | 16-29 | 04:26 | |
| 509 | Chris Carneal Zach Shipps | 40-49 | 04:26 | |
| 509 | Corey Pachciarz Pat Pachciarz | 40-49 | 04:26 | |
| 509 | Adrian Florian Andrea Florian | 16-29 | 04:26 | |
| 509 | Anthony Williams Josh Jang | 30-39 | 04:26 | |
| 509 | Jordan Dunson Spencer Klein | 30-39 | 04:26 | |
| 509 | Etosha Wilson Rico Jordan | 40-49 | 04:26 | |
| 521 | Jesse Foster Kyle Whitmire | 30-39 | 04:27 | |
| 521 | Marc Kenny Casseus Maverick Alcantara | 16-29 | 04:27 | |
| 521 | Bisola Egbe Kevin Drummond | 30-39 | 04:27 | |
| 521 | Glenn Getchell Jeff Mahoney | 50-59 | 04:27 | |
| 521 | Nick Stiles Shea Stiles | 30-39 | 04:27 | |
| 521 | Daniel Lampl Katie Lampl | 30-39 | 04:27 | |
| 521 | Andrea Lowery Kendall Lowery | 30-39 | 04:27 | |
| 521 | Casey Ladisa Josh Tinius | 30-39 | 04:27 | |
| 521 | Adam Leach Danielle Rustem | 30-39 | 04:27 | |
| 530 | Frances Bayly Greg Henrich | 30-39 | 04:28 | |
| 530 | Logan Rohde Monica Rohde | 30-39 | 04:28 | |
| 530 | Alina Varpiotaite Anthony Strayhorn | 40-49 | 04:28 | |
| 530 | Hunter Beuhler Spencer Lowden | 16-29 | 04:28 | |
| 530 | Lauren Green Scott Kerstetter | 30-39 | 04:28 | |
| 530 | Arjun Sawhney Peter Seely | 30-39 | 04:28 | |
| 530 | Brett Spatafore Fan Feng | 30-39 | 04:28 | |
| 530 | Fidel Chavez Jose Chavez | 16-29 | 04:28 | |
| 530 | Jonathan Mock Madison Mock | 16-29 | 04:28 | |
| 530 | Emily Doran Travis Reynolds | 30-39 | 04:28 | |
| 530 | Hillary Evers Matthew Evers | 30-39 | 04:28 | |
| 530 | Nick Barlow Philip Cox | 40-49 | 04:28 | |
| 530 | Chris Francis Kalani Lindsey | 30-39 | 04:28 | |
| 543 | Jacquie Yeh Ken Yau | 40-49 | 04:29 | |
| 543 | Adam Rowe Kyle LaChance | 40-49 | 04:29 | |
| 543 | Marcus Castillo Susan Castillo | 30-39 | 04:29 | |
| 543 | Chris Demoss Danielle Demoss | 16-29 | 04:29 | |
| 547 | Andrew Wallace Klein Hopkins | 30-39 | 04:30 | |
| 547 | Josh Bates Michael Cornett | 30-39 | 04:30 | |
| 547 | Anastacia Castillo Joshua Smith | 30-39 | 04:30 | |
| 547 | Christopher Lacey Madelyn Lacey | 30-39 | 04:30 | |
| 551 | brandon garrett lynsie anderson | 30-39 | 04:31 | |
| 551 | Drew Wansley Heath Beeferman | 50-59 | 04:31 | |
| 551 | brooke teter Daniel patriss | 16-29 | 04:31 | |
| 551 | Minas Lyristis  Philip Neser | 40-49 | 04:31 | |
| 555 | Carter Miller Michael Miller | 16-29 | 04:32 | |
| 555 | Briceton McNair David McMillan | 16-29 | 04:32 | |
| 555 |  Leonardo Paiva Rasoul Zwen | 30-39 | 04:32 | |
| 558 | Colin Congo Laura Congo | 30-39 | 04:33 | |
| 558 | Ainsley Jones David Jones | 30-39 | 04:33 | |
| 558 | Brittany Alexander Scott Ewing | 40-49 | 04:33 | |
| 558 | Paul Jung Terrence Phillip | 16-29 | 04:33 | |
| 558 | Jory Kimbrell Lauryn Davidson | 16-29 | 04:33 | |
| 563 | Michael Abney Sae Park | 30-39 | 04:34 | |
| 563 | Adam Bradley Ryan Mctigue | 30-39 | 04:34 | |
| 563 | Alex Byrd Eddie Nam | 30-39 | 04:34 | |
| 563 | Ashley Elms Hunter Elms | 16-29 | 04:34 | |
| 563 | David Boswell Solomon Cooper | 30-39 | 04:34 | |
| 563 | Charles Kistler Kate Ashmore | 16-29 | 04:34 | |
| 569 | Carson Ruehling Faith Simmons | 16-29 | 04:35 | |
| 569 | Alex Young Darci Davis | 16-29 | 04:35 | |
| 569 | Cassandra Laird Matt Laird | 30-39 | 04:35 | |
| 569 | Ian Goodman Sara Goodman | 30-39 | 04:35 | |
| 573 | Adam Levitt Avery Levitt | 30-39 | 04:36 | |
| 573 | Tori Johnson Tristan Taylor | 16-29 | 04:36 | |
| 573 | John Dooley John Mark Sheffield | 16-29 | 04:36 | |
| 573 | Gerald Dilworth Jr LC Henderson | 16-29 | 04:36 | |
| 573 | Lina Eisa William Everett | 30-39 | 04:36 | |
| 578 | Kendall Lewis Perry Lawson | 30-39 | 04:37 | |
| 578 |  Hanna Blinouskaya Jacob Rasmussen | 16-29 | 04:37 | |
| 578 | Jasmine Macias Julius Gallardo | 16-29 | 04:37 | |
| 578 | Elizabeth Carter Perrin Jones | 16-29 | 04:37 | |
| 578 | Carlo Washington Summerjoy Scott | 30-39 | 04:37 | |
| 583 | Taylor Holder Trevor Vallier | 16-29 | 04:38 | |
| 583 | Angie Callen Gabe Rietveld | 16-29 | 04:38 | |
| 583 | Adelaide Williams James Williams | 40-49 | 04:38 | |
| 586 | Audra McSharry Eric Reece | 16-29 | 04:39 | |
| 586 | Kevin Bar Matthew Ennis | 40-49 | 04:39 | |
| 586 | Greg Welte Nathan Ziegler | 30-39 | 04:39 | |
| 586 |  Carlos Valencia Christen Babcock | 30-39 | 04:39 | |
| 586 | Bridgot Gysbers Duane Gilson | 50-59 | 04:39 | |
| 591 | Christopher Wright Dominique Sheard | 30-39 | 04:40 | |
| 591 | Keith Breneman Richard Rayburn | 40-49 | 04:40 | |
| 593 | Haylen Mann Raul Araya | 16-29 | 04:41 | |
| 593 | Juliette Muller Robert McGovern | 16-29 | 04:41 | |
| 593 | Ajay Handa Sahil Handa | 30-39 | 04:41 | |
| 593 | Regan Sinkuler Robert Hunter | 30-39 | 04:41 | |
| 597 | Ishmael Dean-El Kristin Dean-El | 40-49 | 04:42 | |
| 598 | Brian Leonardo Cadence Campbell | 16-29 | 04:43 | |
| 599 | Al Wolf Yidara Wolf | 30-39 | 04:44 | |
| 599 | Ashley Dix Ulysses Pineda | 16-29 | 04:44 | |
