| 1 |  Andy Bellino Fabian Acuna Hayden Voss Jorge Olivares | 40+ | 03:56 | |
| 2 | Clarence Stanfield Juan Marcano Kendall Sullivan Megan Strickler | U40 | 03:58 | |
| 3 | Cole DeRosa Kelly Sullivan Pamela Fox Todd Wolffrum | 40+ | 03:59 | |
| 4 | Brittany Reed Jana Demmer Kevin Kadak Trevor reed | U40 | 04:01 | |
| 5 | Cristian Mendoza Espinosa Kristin Stitcher Megan Behrle Peter Sandvik | U40 | 04:03 | |
| 6 | Anthony Parascondola Daria Daspin Matthew Kemp Vivian June | U40 | 04:05 | |
| 7 | Jen Stagnoli Meghan McGowan Robert McGowan Sam Brunette | 40+ | 04:11 | |
| 7 | Abigail Giannobile Jessica Gregory Kevin Gregory Zack Wisnowski | U40 | 04:11 | |
| 9 | Alexander Trakhtman Regina Trakhtman Stephanie Sandrino Suk Min Choe | U40 | 04:12 | |
| 10 | Caitlin Kahn John Narducci Julia Macone Stephen Valladares | U40 | 04:13 | |
| 10 | Edward Ham Jeremy Monteiro lauren Raver Megan Monteiro | U40 | 04:13 | |
| 12 | Danielle McCrea Jennifer Rodriguez Joseph Rodriguez Kevin McCrea | U40 | 04:16 | |
| 12 | Alli Vizza Amelia Raese Anthony Vizza Austin Hoover | U40 | 04:16 | |
| 14 | Cristin Barrett Dominic Go Geoffrey Townsend Laura Seymour | 40+ | 04:17 | |
| 15 | Amelia McNamara David Muentes Dorian Williamson Jennalyn Newton | U40 | 04:25 | |
| 16 | David Blum George Yates Sarah Cotter Sarah Normoyle | U40 | 04:26 | |
| 17 | Emily Waddell Jonathan Ladson Luis Gomez Megan Fortier | U40 | 04:33 | |
| 18 | Aaron Bates Anna-Lisa Marcum Butch Leilich Maria Saling | 40+ | 04:35 | |
| 18 | Chad Nevel Erika Shumway Justin Shumway Nicole Nevel | U40 | 04:35 | |
| 20 | Adam Connell Mariel Connell Paul David Glass Shanda Lee | 40+ | 04:36 | |
| 21 | Dalton Kamens Harvyn Valentin Hillary Valentin Sarena Boulier | U40 | 04:37 | |
| 22 | Jennifer Frunza Justin Allen Taylor Casey Victor Frunza | U40 | 04:39 | |
| 22 | Andy Elting Ayden Redmond Desiree Ruiters Tiffany Murphy | 40+ | 04:39 | |
| 24 | Chase Christiansen Colin Christiansen Erika Christensen Nicole Meyer | U40 | 04:40 | |
| 25 | James Lee Jaren Lee Maya Lee Michelle Lee | U40 | 04:42 | |
| 26 | Alexandra Whitford Alvin Huang Gavin Gramstad Hannah Kim | U40 | 04:44 | |
| 26 | Caitlin Lomazzo Chad Zillioux James Cawley Sandi Kinney | 40+ | 04:44 | |
| 28 | Gina Plunto Keith Truitt Shane Plunto Sue Truitt | 40+ | 04:47 | |
| 29 | Jordan Thomas Mia Mozingo Shelby Gonzalez Vanessa Johnson | U40 | 04:48 | |
| 30 | Ethan Goldman Kameron Leardi Leah Ayres Lindsay Collins | U40 | 04:49 | |
| 31 | Gabby Erestain Kris Young Nick Lithisack Vanessa Benitez | U40 | 04:51 | |
| 32 | Caroline Merillat Leslie Carpio Michael Morris Shayne Morris | 40+ | 04:52 | |
| 33 | Alexandra Ruiz Marcus Hempstead Sophie Kim Tyrece Shepherd | 40+ | 04:53 | |
| 33 | Daniel Mifkovic Denise Kuehl Jen Proper Louis Caracci | 40+ | 04:53 | |
| 35 | Austin Lilley Brandon Warren Peyton Eibner Sarah Brennan | U40 | 04:56 | |
| 35 | Hajara Yusuf Kane Samuel Lauren Miller Vaughn Williams | U40 | 04:56 | |
| 37 | Areyal Hall Edward Goins Jonathan Burgess Jr Patrice Malone | U40 | 04:59 | |
| 38 | Brian Betts Carla Castillo Monique Collier Tarik Mills | U40 | 05:00 | |
| 39 | Jeanine Littrel Jim Keenahan Leiaa Batiller Sebastian Oquendo Toro | 40+ | 05:01 | |
| 40 | Angeleaza Anderson Julia Konner Sekou Vaughn Sergio Lizarraga | U40 | 05:07 | |
| 41 | Eleisa Umanzor Eleisa Umanzor Eleisa Umanzor Eleisa Umanzor James Foster James Foster James Foster James Foster Jay Beam Jay Beam Joyce Pang Joyce Pang Joyce Pang Joyce Pang | 40+ | 05:08 | |
| 43 | Aubrey Wells Casper Wells Melissa Bauer Scott Bauer | U40 | 05:10 | |
| 44 | Alexia Mitchell Ian Thorne Luis Arteaga Monique Brooks | U40 | 05:13 | |
| 45 | Jennifer Baker John Alukonis Lindsay Alukonis Warren Jackson | 40+ | 05:18 | |
| 46 | Brian Stiles Emmanuel Bockarie Jennifer Medina Sarah Bockarie | U40 | 05:19 | |
| 47 | Brian Trainer Elisa Rodriguez Hunter Tressler Rachael Weaver | U40 | 05:20 | |
| 48 | Courtney Purtell Evan Ostrow James Purtell Nicole Purtell | U40 | 05:21 | |
| 49 | Elizabeth Soong Luis Soriano Mamee Groves Rusty Austin | 40+ | 05:26 | |
| 50 | Jen Meehan Kane McGovern Kara Mueller Thomas Garlock | U40 | 05:28 | |
| 50 | Bryan LaRosa Clarence Stevens Jessica Gibian Sydney McKinley | U40 | 05:28 | |
| 52 | Andrea Bennett Benjamin Blackwood Daniel Schaal Kristen Blackwood | 40+ | 05:29 | |
| 53 | Danielle Byrd Jacob Mosh Jason Volpicelli Nicole Hunter | U40 | 05:33 | |
| 53 | Carla Johnson Guillermo Leon Stephen Yancey Syreeta Jackson | 40+ | 05:33 | |
| 55 | David Shoyombo Justin Pearson Maya Kim PJ Fiore | U40 | 05:34 | |
| 56 | Alexis Jackson Brianna Perry-Jackson Devonte Jackson Herbert Jackson | U40 | 05:40 | |
| 57 | Alexander Pytlar Amy Saxton Hannah Smith Joon Chung | U40 | 05:42 | |
| 58 | Alicia Crump Chris C Derrick D Manisha Pal | U40 | 05:56 | |
| 59 | Alitza Vega Blanca Campa Gabriel Botero Jr Gabriel Mendez | U40 | 05:57 | |
| 60 | David Torain Manal Maarouf Monica Iturbide Paul YU | 40+ | 06:02 | |
| 61 | Andre Robinson Krystina Robinson Marine Langreney Richard Shinners | U40 | 07:03 | |
| 62 | Jery Huntley Kat Torre Mike Cherubin Nick Wallace | 40+ | 08:47 | |
