| 1 |  Dennis Soelle  Seka Arning | 30-34 | 03:37 | |
| 1 | Dan BUCKINGHAM  Sandy VO | 30-34 | 03:37 | |
| 3 | Charlie Cullen Rachel CLOUSING | 30-34 | 03:38 | |
| 4 | Ellie TURNER Justin MEDEIROS | — | 03:39 | |
| 5 |  Dena Hogan Tom Hogan | 40-44 | 03:40 | |
| 6 | Chase Smith Natalie BRADLEY | 30-34 | 03:44 | |
| 6 | Justin GIBBS Karly VICINI | 30-34 | 03:44 | |
| 8 | Dominique Sottile Matthew SCRIVNER | — | 03:45 | |
| 9 | Jana Lebenstedt Patrick Martens | 30-34 | 03:47 | |
| 10 | Emily Rethwill Phillip MUSCARELLA | 30-34 | 03:48 | |
| 10 | Lauren ASPLIN Richard MUNCHOW | — | 03:48 | |
| 12 | Alec SCHNITZLER Olivia MARCHESCHI | 30-34 | 03:49 | |
| 12 | Alex Miller Zoe Verkerke | 30-34 | 03:49 | |
| 12 |  Alex Beran Tyler GANLY | 30-34 | 03:49 | |
| 12 | Brian SEGHERS Terra Jackson | 30-34 | 03:49 | |
| 12 | Chelsie Grant Mike Zuriek | 30-34 | 03:49 | |
| 17 | Andrea Duncan Taylor HANEY | 30-34 | 03:50 | |
| 17 | Dustin DIEFENDERFER Lindsey DIEFENDERFER | 40-44 | 03:50 | |
| 17 | Teresa PÖTING Thorsten HÜTZ | 30-34 | 03:50 | |
| 17 | Kelly TULAY Ryan TULAY | 30-34 | 03:50 | |
| 21 | Ali Summers Shawn BOOTH | 30-34 | 03:51 | |
| 21 | Gabriela Olivier Gerrel OLIVIER | 30-34 | 03:51 | |
| 23 | Rachel NOEL Tanner STAHL | — | 03:52 | |
| 24 | Hannah COOK Robert PARMAN | — | 03:53 | |
| 24 | Ashley HOGAN Chris HOGAN | 30-34 | 03:53 | |
| 24 | Ashton JEFFREY Jodie BAKER | — | 03:53 | |
| 27 | Cedric GODDEVRIND Julianne FISHER | 30-34 | 03:54 | |
| 27 | Ali RICE Wyatt AZEVEDO | — | 03:54 | |
| 27 | Nik GILLIHAN Pilar FISK | — | 03:54 | |
| 27 | Megan GREENER Ryan Johnson | — | 03:54 | |
| 31 | Kendra COWLEY Tate COWLEY | 30-34 | 03:55 | |
| 31 | Daniel WOLF Jessica GARCIA | 30-34 | 03:55 | |
| 31 | Jeremy FLINTER Marisa TREMBLAY | 30-34 | 03:55 | |
| 31 | Dominic Popolizio Nicole Popolizio | 30-34 | 03:55 | |
| 31 | Angela LEIDER Jeff LEIDER | 30-34 | 03:55 | |
| 36 | Brandon Hughes Maria COLACURCIO | 40-44 | 03:56 | |
| 36 | Abigail YATES Harvey YATES | 30-34 | 03:56 | |
| 36 | Hunter FARIS Lauren OGDEN | 30-34 | 03:56 | |
| 36 | Kelli WATKINS Matthew WATKINS | 30-34 | 03:56 | |
| 36 |  Alex Meyers Christopher Meyers | — | 03:56 | |
| 41 | Kristyn WILBANKS Ryan MAGUIRE | — | 03:57 | |
| 41 | Chelsie GRANT Mike ZUREK | 30-34 | 03:57 | |
| 41 | Ashley YATES Rilyn LEWCHUK | 30-34 | 03:57 | |
| 41 | Devin THOMAS Megan ESCOBAR | 30-34 | 03:57 | |
| 41 | Jessica BELL Troy STEVENS | — | 03:57 | |
| 41 | Dennis LESNIAK Erica CASO | 30-34 | 03:57 | |
| 47 | Courteney Landis Louie GERMAIN | — | 03:58 | |
| 47 | Ellie DUNLAP Nate AMBROSINI | — | 03:58 | |
| 47 | Deml Benson Klyson KAIWI | — | 03:58 | |
| 47 | Hannah THOMAS Rob THOMAS | 30-34 | 03:58 | |
| 47 | Austin PHILBIN Sarah PHILBIN | 30-34 | 03:58 | |
| 47 | Celene BATREZ-SCOTT Jacob SCOTT | 30-34 | 03:58 | |
| 47 | Daniel Weymier Lindsey ROSE | 30-34 | 03:58 | |
| 47 | Adam HAWES Miranda O'NEILL | 30-34 | 03:58 | |
| 47 | Ivan RADOVANOVIC Xanthinna MCMAHON | 30-34 | 03:58 | |
| 47 | Grady SMITH Susan FRIEDLANDER | 30-34 | 03:58 | |
| 57 | Jenna REID Sam TOOLEY | — | 03:59 | |
| 57 | Julia KACZMAREK Steve GOETZ | 30-34 | 03:59 | |
| 57 | Alex TEMPLE Jennifer TEMPLE | 30-34 | 03:59 | |
| 60 | Jess CONTRERAS Lorenzo Contreras | 40-44 | 04:00 | |
| 60 | Bailey COOK Davey FERNANDEZ | 30-34 | 04:00 | |
| 60 | Braydon LOPEZ Taylor GARZA | — | 04:00 | |
| 60 | Sheridan METCALF Timothy PACATTE | 30-34 | 04:00 | |
| 60 | Brittany CRAWFORD Peter NGUYEN | 30-34 | 04:00 | |
| 60 | Clare DIMOND Desmond DIMOND | 30-34 | 04:00 | |
| 60 | Konnor FLEMING Shania FLEMING | — | 04:00 | |
| 60 | Taryn Titus Weston TITUS | 30-34 | 04:00 | |
| 60 |  Kevin CERAN JERUSALEMY Mehiti WANE | — | 04:00 | |
| 69 | Chaz Donati Semaj Walker | — | 04:01 | |
| 69 | Abbey GENTRY Jason GENTRY | 30-34 | 04:01 | |
| 69 | Doanh WRIGHT Willie WRIGHT | 30-34 | 04:01 | |
| 69 | Robbie ZICARI Shyla DAVENPORT | — | 04:01 | |
| 69 | Kristin WHITESIDE Lalo CONTRERAS | 30-34 | 04:01 | |
| 74 | Brian WILKERSON Lindsay BALKENBUSH | — | 04:02 | |
| 74 | Jess LANCASTER Reece FALLON | 30-34 | 04:02 | |
| 74 | Afiya LETFORD James ENGLISH | — | 04:02 | |
| 74 | Emily BRINEGAR Kole BRINEGAR | 30-34 | 04:02 | |
| 74 | Marika HAMEL Rock LANGLOIS | 30-34 | 04:02 | |
| 74 | Craig QUIGLEY Tiffany THEOPHIL | 30-34 | 04:02 | |
| 74 | Christian MARRINER Madison DAGEN | 30-34 | 04:02 | |
| 74 | Andrew THOMAS Sabrina BRELLISFORD | — | 04:02 | |
| 74 | Deshawn JORDAN Kristina JUSTINIANO | 40-44 | 04:02 | |
| 83 | Jess Pettrow Ted PETTROW | 30-34 | 04:03 | |
| 83 | Chad DILZER Lindsay PICONE | 30-34 | 04:03 | |
| 83 | Brian BRENZEL Kerrianne BRENZEL | 30-34 | 04:03 | |
| 83 | Ian DOIRON Whitney DOIRON | 40-44 | 04:03 | |
| 83 | Ellen MOISSEYKIN Vlad MOISSEYKIN | 30-34 | 04:03 | |
| 83 | Jacob WALKER Meagan WALKER | 30-34 | 04:03 | |
| 83 | Christina OSWALD Spencer OSWALD | 30-34 | 04:03 | |
| 83 | Joshua GRAFFAM Ruth GRAFFAM | 40-44 | 04:03 | |
| 83 | Brooke FLOYD Erik HUDSON | 40-44 | 04:03 | |
| 83 | Deserei BECKHAM Zackary BECKHAM | 30-34 | 04:03 | |
| 83 | Chris ECKSTRUM Jenna ECKSTRUM | 30-34 | 04:03 | |
| 83 | Eric WULFF Karen LOAIZA-WULFF | 30-34 | 04:03 | |
| 95 | Cooper MILTON Taylor BEGEMANN | — | 04:04 | |
| 95 | Collette WEST Jose BRITO | 30-34 | 04:04 | |
| 95 | Brandon Freeman Jensen BRADFIELD | — | 04:04 | |
| 95 | Emily HAAN Tommy CICHA | — | 04:04 | |
| 95 | Jazmin PATTIWILA Rick REYNOSO | 30-34 | 04:04 | |
| 95 | Devin JOHNSON Marielle SCHULTZ | 30-34 | 04:04 | |
