| 1 |  Dylan Scott  James Muscart Sarah Hernandez Terra Jackson | U40 | 03:56 | |
| 2 | Abigail Leone Joey Quinones Matthew Gelchion Megan Voigt | U40 | 04:09 | |
| 3 | Brenner Swenson  Gisela Miranda Difini Thomas Schuler Miller Veronica Leynes-Zwirn | U40 | 04:11 | |
| 4 | Larissa Reinkensmeyer Paula Gutiérrez Raymond Melencio Shaun Baluyot | U40 | 04:12 | |
| 5 | Ben Zeeb Brian Cunningham Jen Stagnoli Stephanie Ricedorf | 40+ | 04:18 | |
| 6 | Ashley Etue Carina Burdick Karl Heuchert Michael Jeng | U40 | 04:33 | |
| 7 |  Colm Buckley Emma Roberts Maria Rueda Timothy Vella | U40 | 04:37 | |
| 8 | Ariel Brenits Jenny Scherer  Prabhath Kiran Atmakuri Stefan Hench | U40 | 04:51 | |
| 9 | Kerry Hughes Lacy Marinenko Todd Loesch Vitaly Slabada | 40+ | 04:52 | |
| 10 | Brian Reindl Jamie Enderby Tiffany Agamaite Zachary Enderby | 40+ | 05:10 | |
| 11 | Jake Schoolmeesters James Wright Janina Dunklau Keyshlamarie Pérez Muriel | U40 | 05:16 | |
| 12 | Dionis Harvey Kendall Patterson Mekeya Brown Myshawn Smith | U40 | 05:19 | |
| 13 | Danielle Schiff Ernest Sherman Mike Childers Nancy Witt | 40+ | 05:22 | |
| 14 | Andreina Damiano Chelsey Bazuro Colin Parker Daniel Castro | U40 | 05:56 | |
| 15 | Ali Barzyk David Webster Jordan Harris Tammy Nguyen | U40 | 06:06 | |
