| 1 |  Christopher De Klerk Kweku Bennin Melissa Reyneke Rizqah Van Der Schyff | U40 | 03:58 | |
| 2 |  Danielle Crewe George Crewe Keegan Peach Kerri Huntington | U40 | 04:01 | |
| 3 | Brent Shone David Frank Demi Joseph Erin Goodman | U40 | 04:04 | |
| 4 | Courtney Marais Nazier Mohamed Richard Mcenery Roxanne Fairley | U40 | 04:10 | |
| 5 | | U40 | 04:12 | |
| 6 | Andrew Proudman Eric Fourie Hitge Roxy Louw Tammy Jacobs | U40 | 04:22 | |
| 7 | Caryn Cyster Giovanni Carelse Jesse-Mari Solomons Reagan Adams | U40 | 04:24 | |
| 8 | Gouwa Jacobs Hoosain Lalkhen Sulaiman Jakoet Yasmeen Lunat | U40 | 04:27 | |
| 9 | Daniel Scheepers Frannie Langenhoven Marina Van Tonder Salomi Dreyer | U40 | 04:31 | |
| 10 | Aya Jolobe Cameron Heynes Jesse-Claire Speller Mandy Meltz | U40 | 04:41 | |
| 10 | Daniela Carapeto Jason Preto Kumien Senekal Paul Elkington | U40 | 04:41 | |
| 12 | Danielle Jordaan Lucinda Bosch Ricardo Lategan Wynand Maclachlan | U40 | 04:42 | |
| 13 | Andrea Scheepers  Cornelia Karlsson Jarrod Loubser Zac Lewis | U40 | 04:52 | |
| 14 | David Sher Jordyn Stoos Samantha Calitz Sinethemba Ceba | U40 | 04:54 | |
| 15 | Cameron Cleaver Joanne Keyser Lida Versfeld Menzi Ndhlovu | U40 | 04:57 | |
| 16 | Chris Moulam-Jones  Holly Archer  Imke Salander Joe Greenslade-Cross | U40 | 04:58 | |
| 17 | Alistair Simpson Hilary Hefer Noah Hefer Samuel Senekal | U40 | 05:01 | |
| 18 | Hugo Van Niekerk  Jeffrey Schnabolk Leo-Marie Grobler Naomi Bruwer | U40 | 05:04 | |
| 19 |  Alice Beattie Bronwen Seager Jay Hackart John Rostance | U40 | 05:06 | |
| 20 | Alex Campbell Leon Redelinghuys Marsha Adonis Suzél Vosloo | U40 | 05:09 | |
| 21 | Crystal Espin Genevieve Morgan Thabit Ishmail Tyler Kendall | U40 | 05:14 | |
| 22 | Alexa Schempers Dwayne Hurn Gareth Mcgregor-Langley Phumi Khumalo | U40 | 05:15 | |
| 23 | Alita De Witt Jp Steyl Luthando Basela Rachel Joffe | U40 | 05:18 | |
| 24 | Ettienne De Lange Jodiane Benade Leandro Rogers Taryn Alexander | U40 | 05:29 | |
| 25 | Barbie Erasmus Daniel Willemse E-Andri Swiegelaar Joel Erasmus | 40+ | 05:51 | |
| 26 | Corlia Meyer Greta Marie Dönnecke Lorena Tejera Mannino Sachin Bam | U40 | 06:04 | |
