| 1 |  Ben Sutherland  Lauren-Leigh Richardson  Lauren Stockley Travis Owles | U40 | 03:41 | |
| 2 | Alexander Eyre Amy Walker Cameron Burrows Shaunie Gwen Gibson | U40 | 03:55 | |
| 3 | Brendan Colls  Courtney McAlister Grant Major Holly Whetnall | U40 | 03:56 | |
| 4 | Louise Varney Michael Price Richard Prendergast Tammy Price | 40+ | 04:00 | |
| 5 | Davyd Parry Lindsay Murphy Naomi Lawrence Tom Perry | U40 | 04:05 | |
| 5 | Chloe Attwal Chris Grundy Craig Owen Faye Haddon | U40 | 04:05 | |
| 7 | Jessica Jordan Megan Daley Robert Sonczak Tom Daley | U40 | 04:06 | |
| 8 | Andy Taylor Emma Moffat Louise Crooks Luke Tarbatt | 40+ | 04:09 | |
| 9 | Henry Moriarty Laura Wordley Rhiannon Kenny Rob Taylor | U40 | 04:10 | |
| 10 | Andrew King Mya Crowder Nicole Fenwick Sam Askew | U40 | 04:11 | |
| 11 | Andrea Richardson Carl Woodward John Fairbrother Kelly Bretherton | 40+ | 04:12 | |
| 12 | Dean Gillson Holly Gillson Jessica Bambrook Simeon Bambrook | U40 | 04:14 | |
| 13 | Ian Nichol Lauren Smith Mica Picken Thomas Dawson | U40 | 04:16 | |
| 14 | Maddie Bell Matt Furlong Oliver Powell Rowanna Hughes | U40 | 04:17 | |
| 14 | Gemma Lewis Kris Woollard  Maxine Owen Paul Lewis | 40+ | 04:17 | |
| 16 | Jim Wylie Nikki Lambert Rebecca Bostock Slav Wojcik | 40+ | 04:21 | |
| 16 | Callum Lee Jamie Penton Leah Hopton Mahala Pettit | U40 | 04:21 | |
| 16 | Ben Brogden Jack Hallett Jasmine Anthony Kimberley Walker | U40 | 04:21 | |
| 19 | Annie Salter Jack Smith Katy Smith Steve Copsey | U40 | 04:24 | |
| 20 | Dan Putnam Jessica Tappin Marcus Dowdeswell Molly Tappin | U40 | 04:25 | |
| 21 | Liam Clark Liam Slater Natalie Dunning Rebecca Hunt | U40 | 04:28 | |
| 22 | Charlotte Moore Hollie Lea Jake Ford Louie Parish | U40 | 04:29 | |
| 23 | Ashlea Mills Christopher Mills Jacob Wood Sophie Newman | U40 | 04:30 | |
| 24 | Andrew Cawthray Emma Rai Rebecca Loran Sean Rai | U40 | 04:32 | |
| 25 | Jake Wilkes James Melhuish Kate Wild Paige Atherton | U40 | 04:33 | |
| 25 | Chris Mcelwee Jo Faulkner Natalie Bond Paul Simpson | 40+ | 04:33 | |
| 27 | Hayley Carter Max Tyrer Russell Green Sophie Carter-Steele | U40 | 04:34 | |
| 28 |  Dzenana Kulenovic John Sullivan Mariah Anwar Nedjad Mehdic | 40+ | 04:38 | |
| 29 | Emily Harris Euan Whitbourn Jess Gilbert Lewis Miles | U40 | 04:39 | |
| 30 |  Adam Worotynski Ashley Lapidge Danielle Chechlacz Grace Smeaton | U40 | 04:42 | |
| 31 | Alexander Cleves Hannah Cleves Jemma Lees Tom Johnson | U40 | 04:44 | |
| 31 | Ben Rudd Helen Channon Stuart Channon Tammy Rudd | U40 | 04:44 | |
| 33 | Danielle Baylis Jennifer Munslow  Johnathan Stephen-Smith Paul Hartley | 40+ | 04:45 | |
| 34 | Fiona Rose James Rose Samantha Johnson Tim Morgan | 40+ | 04:46 | |
| 35 | Alexander Ahmed Anjul Chandi Joanna Tague Mickey Chandi | U40 | 04:54 | |
| 35 | Carmen Powell Charlie Chaplin David Cottrell Sharon Houlton | 40+ | 04:54 | |
| 37 | Caroline Metcalfe Hannah Kay Luke Pulleyn Richard Chandler | U40 | 04:58 | |
| 37 | Becky Busby James Hammond Lucie Gray Phillip Busby | U40 | 04:58 | |
| 39 | Harvey Talwar Louise Nott Nada Frayne  Tony Nguyen | U40 | 05:05 | |
| 40 |  Adebisi Akinlabi Adebola Ayinde Ibitoye Ibikunle Moses Ayinde | U40 | 05:07 | |
| 41 | Brian Pearson Emma Wood Maryla Nowakowska Tom Pearson | 40+ | 05:22 | |
| 42 | Eric Nagel  Lucy Williams Rosalia Padron Pol Sonny Chapman | U40 | 05:24 | |
| 43 |  Fabio Verdicchio Ingrid Taylor Mark Gauci  Marlene Lowe | 40+ | 05:36 | |
| 44 | Giussepe Mora  Maxime Bonelli Natalie Piper Zainab Hakim | U40 | 05:37 | |
| 45 | Anton Inckle James Pountney Jenni Wilkes Liz Delaney | U40 | 05:41 | |
| 46 | Emily Johnson Joshua Haigler Petra Hewitson Timothy Owen | 40+ | 05:44 | |
| 47 | Belijeet Daffu Henry Nandra Laura Smith Shin Ark | 40+ | 05:51 | |
| 48 | Dominic Phillipa Kyra Woodings Maddie Locke Zak Taylor | U40 | 05:52 | |
| 49 | Ben Broome Claire Broome Holly Clifton Karl Frew | 40+ | 05:55 | |
| 50 | Alex Dunne Emma Mahon Olivia Mahon Romario Dunne | U40 | 06:07 | |
| 50 | Amber Jewell Emma Handley Jordan Wheatley Luke Edwards | U40 | 06:07 | |
| 52 | Carla Elwell Craig Coughtrey Lesley Hingley Luke Harris | U40 | 06:33 | |
| 53 | Aaron Collins Iona McCormick Isla McCormick Justin Mccormick | U40 | 08:45 | |
