| 1 |  Ahmee Al-Hendi  Hannah Woolley Jacqueline Laferriere  Ryan Wren | U40 | 03:57 | |
| 2 |  Alexander Golik Amber Tait Chelsie Grant Zach Tait | U40 | 03:58 | |
| 3 | Annie Gordon Katrina GOBEILLE Mitchell Creasey Shane Lloyd | U40 | 04:01 | |
| 4 | Braden Usher Cameron Demeule Fiona Begg Mary Begg | U40 | 04:04 | |
| 5 | Andreas Axiotis Holly Bennett Julie Frias Tyler Bennett | U40 | 04:06 | |
| 6 | Cam Dewar Markus Molder Rasa Augaitis Talia Barron | U40 | 04:08 | |
| 7 | Jaimy Hunt Matthew Johnson Rachel Teoh Rob Kumer | 40+ | 04:11 | |
| 8 | Christian Isleta Dexter Jao Kimberly Bunardi Lesley-Anne Isleta | U40 | 04:13 | |
| 9 | Eoghan Mcmullen  Frederik Brudy Melinda Lo Neda Mcmullen | U40 | 04:18 | |
| 10 | Dennis Clyne Ida Harkness Jason Chamberlain Kelly Martin | U40 | 04:19 | |
| 11 | Brittany Marriott Chris Christensen Meaghen Garrett Mitchell Mclaughlin | U40 | 04:21 | |
| 12 | David Garnis Dianna Leroux Jessy Garnis Stephen Harrison | 40+ | 04:23 | |
| 13 | Dre David Jamie Lee Rodd BM76 Shelby Hall | 40+ | 04:25 | |
| 14 | Allison Marcial  Bradley Marcial Ivy To Kai Zhou | U40 | 04:27 | |
| 15 | Douglas Lam  Henry Venegas Segura Leann Lapp Stephanie Boikovitis | U40 | 04:29 | |
| 15 | Ashley Nardell Cheyenne Russell Daniel Vanin Michael Irvine | U40 | 04:29 | |
| 17 | Patrick Zenta Samantha Bauer Stefanie Ellul Umair Saleem | 40+ | 04:30 | |
| 18 | Aaron Chia Kaitlyn Weyersberg Kyle Alvez Victoria Chia | U40 | 04:31 | |
| 18 | Chris Badibengi Emma Russo Monique Christie Rahim Karmali | U40 | 04:31 | |
| 20 | Adam Piatkowski Cristina Bregar Juan Macario Roxy Chis | U40 | 04:33 | |
| 21 | Ashleigh Stevenson Courtney Roedding Mark Ritcey William Hall | U40 | 04:36 | |
| 22 | Bryan Snow Geoff Morston Joy Alma Renée Heintz | 40+ | 04:42 | |
| 23 | Haifa Al-Nahdy Hailee Westney Madhu Vijayan Mazdak Mirabi | U40 | 04:43 | |
| 24 | Claudia Torres Mike Lee Simran Sandhu William Logar | 40+ | 04:46 | |
| 25 | Dave Vellenga James Power Nicole Goodbrand Nikki Peyrovan | U40 | 04:47 | |
| 26 | Dominic Abogado Gabrielle David Lydia Palmer Wesley Cheung | U40 | 04:54 | |
| 27 |  Erika Cardenas  Mehrdad Solhi Miraluna Nieles Vladimir Li | U40 | 04:56 | |
| 28 | Drew Dalius Kelly Dalius Timothy Mazur Vicky Hill | U40 | 05:00 | |
| 29 | Alanna Norris Art Lai Sabrina Chan Elan Bibas Kristoffer Laser Moraleja | U40 | 05:03 | |
| 30 | Aliyah Mohamed Cocoro Mori Joe Greenman Warren Saldanha | U40 | 05:21 | |
| 31 | Armando Reyes Emma Whitehall Marco Pierini Vincenza Musicco | U40 | 05:24 | |
| 31 | Azie Lighvani Babak Fahmi Martell Hosseini Shabnam Moier | 40+ | 05:24 | |
| 33 | Chelsea Lam Joel Tellier Keith Porter Natalie Liconti | U40 | 05:25 | |
| 34 | Evan Hills Lauren Hills Madison Carvalho Matt Rodgers | U40 | 05:34 | |
| 35 | Brandon Strazzella Jessica Simpson Lauren Coyle Nicolaas Earley | U40 | 05:35 | |
| 36 | Daniella Avidago Ellen Fielding Mike Zurek Wayne Burns | U40 | 05:38 | |
| 37 | Edward Ng Jesse Levy Michelle Carroll Sean Kidd | 40+ | 05:41 | |
| 38 | Giovanna Fariello Rob Hemington Sarah-Claire Campbell Scott Forester | 40+ | 05:44 | |
| 39 | Izabella Jeffers Michelle Salamillas Paul Andersen Robert Salamillas | 40+ | 05:47 | |
| 40 | Annette Santilli Dorijan Music Lauren Botnick Ted Grigoratos | U40 | 05:53 | |
| 41 | Deborah Huys Ed Tse Kathryn Sheridan Matthew Pym | 40+ | 06:12 | |
